A rapid protein folding assay for the bacterial periplasm
نویسندگان
چکیده
منابع مشابه
Folding of a bacterial outer membrane protein during passage through the periplasm.
The transport of bacterial outer membrane proteins to their destination might be either a one-step process via the contact zones between the inner and outer membrane or a two-step process, implicating a periplasmic intermediate that inserts into the membrane. Furthermore, folding might precede insertion or vice versa. To address these questions, we have made use of the known 3D-structure of the...
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متن کاملReview Protein quality control in the bacterial periplasm
The proper functioning of extracytoplasmic proteins requires their export to, and productive folding in, the correct cellular compartment. All proteins in Escherichia coli are initially synthesized in the cytoplasm, then follow a pathway that depends upon their ultimate cellular destination. Many proteins destined for the periplasm are synthesized as precursors carrying an N-terminal signal seq...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2010
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.388